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NH DyeAGNOSTICS (NHD) sees a steadily increasing demand on its Protein Services. Beside the identification of potential biomarkers, there is increasing demand on Host Cell Protein analysis as well as the identification of differentially modificated proteins (e.g. Glycosylation).
A key role is NHDs long-term experience in sample preparation especially when it comes to more challenging samples. In addition our well focussed analysis strategies ensure rapid and most valuable results.
Smart Protein Layers (SPL) is a standard-based technology for stain-free, quantitative and standardized analysis of proteins separated by 1D gel electrophoresis. SPL is based on rapid fluorescent labeling of protein samples using Smart Labels and a bi-fluorescent standard, the Smartalyzer (SMA).
The relation between fluorescent labeled protein sample and the SMA allows highly sensitive, stain-free protein visualization as well as for precise protein normalization and quantification.
In this paper we demonstrate that the SPL technology allows for absolute protein quantification even if varying amounts of protein have been applied to the gel - either by variations in the protein content or by loading different amounts of sample.
The patented SPL technology is designed for stain-free, qualitative and quantitative protein analysis in protein gels ... learn more here
... as well as to co-detect sample protein on Western Blots while the target is detected (reliable Western Blot normalization) ... learn more here.
Ich danke allen unseren Kunden und Partnern und natürlich unseren Mitarbeitern für das für uns wieder sehr erfolgreiche vergangene Jahr.
Für das Jahr 2018 wünsche ich Ihnen und uns Glück und Gesundheit, berufliche und persönliche Erfolge, Zeit und Kraft für alles das, was aber auch noch im Leben wichtig ist.
Dr. Jan Heise
NH DyeAGNOSTICS GmbH
NH DyeAGNOSTICS is partnering the Scientific Meeting
N-term 2017: Proteostasis via the N-terminus
September 11-13th 2017
located at the Leibniz-Institute of Plant Biochemistry in Halle, Germany.
List of speakers
Christopher Brower, Nico Dissmeyer, Mark Ditzel, Jürgen Dogmen, David Dougan, Ruth Geiss-Friedlander, Kris Gevaert, Daniel Gibbs, Carmela Giglione, Emmanuelle Grafite, Richard Fahlman, Emily Flashman, Michael Holdsworth, Yong Tae Kwon, Dominique Leboeuf, Francesco Licausi, Thierry Meinnel, Angelika Mustroph, Jos Schippers, Hyun Kyu Song, Takafumi Tasaki, Frederica Theodoulou, Klaas van Wijk, Rens Voesenek, Markus Wirtz, Martin Zenker
The group of the organizer, Dr. Nico Dissmeyer has successfully used the patented SPL technology for quantitative Western Blot analysis (Faden et al. 2016*).
Abstract of our meeting poster:
Real-time Normalization of Western Blots: Smart Protein Layers
Jana Heise1,2, Jan Heise1
1 NH DyeAGNOSTICS GmbH, Weinbergweg, 23 06120 Halle, Germany
2 Weinberg Laboratories, Weinbergweg 23, 06120 Halle, Germany
Over decades, Western blotting is a method to detect a target protein in a complex sample. The technique includes protein separation, transfer to a membrane and several washing and antibody incubation steps.
Reliable comparison of detected target signal intensities between different samples is still a problem. It requires an appropriate way of normalization to assure that the reported fold changes of the target protein are not an artifact of a reference signal and/ or experimental errors.
Smart Protein Layers (SPL) is a add-on kit for the detection of sample proteins present on the blot at the same time the target is immuodetected. It is based on two components: i) fluorescent SPL Labels, bound to the sample protein prior separation, for the visualization of all proteins present on the blot at this time ii) bi-fluorescent SPL Standards to monitor labeling efficiency and to provide additional information about the sample and to enable automated data evaluation.
With SPL the determined expression of a specific target protein can be normalized to its corresponding sample, providing a reliable and precise tool for the comparison of fold changes between different samples.
*Faden F., Eschen-Lippold L., Dismeyer N..2016. Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling. Methods Mol Biol. 1450:247-58.
Are you looking for a powerful replacement of EXCEL gels?
Do you analyze more than 8-10 samples?
Or do you want to apply more volume than 12-15 µl per slot?
The new VELUM Precast 1D gels offer
• new: even better resolution
• new: 25 slots á 25 µl sample volume
• new: load and run - buffer free system
• suitable all for protein stains (e.g. Coomassie/ Silver)
• enabled for stain-free, standardized fluorescent standard based SPL technology