- February 2020
- June 2019
- May 2019
- November 2018
- September 2018
- June 2018
- April 2018
- January 2018
- December 2017
- September 2017
- August 2017
- June 2017
- March 2017
- September 2016
- February 2016
- September 2015
- February 2015
- January 2015
- October 2014
- June 2014
- April 2014
- March 2014
- November 2013
- July 2013
- June 2013
- April 2013
- March 2013
- February 2013
- October 2012
- September 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- December 2011
- September 2011
- August 2011
- May 2011
- January 2011
In addition to its powerful gel-based protein and proteome services NHD offers sophisticated mass spectrometry (MS) services such as
- • Shot Gun MS for protein identifcation in proteomes
- • Targeted MS for ID and validation of biomarkers
- • MALDI-TOF fingerprinting
NHD mass spec services enables the detection, identification and quantification of proteins by measuring their mass and characterizing their chemical structure.
Mass spectrometry in proteomics
In proteomics, mass spectrometry is applied to characterize and sequence proteins. Over the last decades, it has become an indispensable technique. Its development over the last decades has led to three fundamental applications: determining protein interaction, categorizing protein expression and locating protein modification.
Mass spectrometry in pharmacology
In pharmacology, mass spectrometry is applied for drug discovery to determine the structure of drugs and metabolites and for screening metabolites in biological systems.
Tandem Mass Spectrometry (MS/MS)
Tandem mass spectrometry involves a tandem mass spectrometer. The sample undergoes ionization and mass-charge ratio separation in the first stage of mass spectrometry (MS1). Then, selected ions of the articular mass-charge ratio are fragmented to generate product ions for detection in the second stage of mass spectrometry (MS2).
Tandem mass spectrometry gives additional information about the selected ions. It is typically applied to sequence proteins and oligonucleotides because the fragments analyzed can be compared to peptides or nucleic acid sequences found in databases.
ORCA Multitasking Workstation
The ORCA Multitasking Workstation brings gel-based electrophoresis to the modern digital life.
No time-consuming staining/ destaining of gels but fast automated read-out, analysis and reporting within seconds.
The system provides you an ideal replacement for the former PhastSystem from GE Healthcare.
Learn more about System Integrated Digital Electrophoresis
Monitoring and Normalization of Sample Protein
The immunochemical detection of specific proteins by Western Blot analysis is performed by different steps including: protein extraction, separation, transfer, blocking, antibody incubation, washing, target detection.
The protein transfer rate is less than 100%. Transferred proteins are not covalently bound to the blotting membrane. Therefore, the amount protein of each sample is continuously decreased (see figure below). The loss of protein is caused by different sources (e.g. hydrophobicity, mechanical shearing forces,...).
Valid Normalisation of Western Blots
The patented Smart Protein Layers (SPL) technology offers you the easy but sensitive detection of sample protein during all stages of the Western Blot procedure. The precise detection of sample amount present on the blot at the same time the target is immunodetected allows for the accurate normalisation of target protein expression.
Patented SPL Technology
The corresponding SPL kit comes with fluorescent SPL label which rapidly bind to the sample protein prior electrophoresis. Depending on sample properties two different workflows can be used.
SPL EASY workflow
Very easy usage of the SPL kit limited to samples within a range of 25μg - 75μg of extracted total protein.
SPL ADVANCED workfow
Advanced usage of the SPL kit for scarce samples and/ or particular buffer compositions. This approach provides further information about the sample, the labeling reaction and monitors the complex WB workflow.
Both the easy and advanced workflow offer precise protein transfer monitoring and allow for experiment-to-experiment comparisons.
Order your SPL kit now!