Refraction-2D™ Labeling Kits

Refraction-2D™ is the leading technology for multiplex-fluorescence 2D gel electrophoresis (also known as differential gel electrophoresis; 2D DIGE). It is based on irreversible binding of new high performance fluorescent G-Dyes (blue G-Dye100, green G-Dye200, red G-Dye300 and infrared G-Dye400) to lysine in proteins.

The G-Dyes are outstandingly photostable and can be re-imaged over periods of months. Thanks to a special low molecular weight G-Dye minimal-labeled proteins can be picked directly from the 2D gel – no further staining required.

Labeling your samples with Refraction-2D™ allows you to...

• •  run up  to 4 different samples within one 2D gel 
• •  detect proteins with abundance as low as 0.03 ng
• •  discover even minor but important protein regulation
• •  identify post translational protein modifications
• •  pick spots directly from the gel, no post-staining required
• •  expert support by our 2D coaches

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We offer state-of-the-art 2D software Delta2D for the analysis of Refraction-2D™-labeled proteins. It provides sophisticated and quick image processing and analysis. Other 2D software are also compatible with Refraction-2D labeling e.g., SameSpots (Nonlinear Dynamics), DeCyder (GE Healthcare), PD Quest (Biorad). 

Rapid and sensitive G-Dye image acquisition can be perforemd with the Octoplus QPLEX (RGB-IR, ECL). 

Our labeling kits come in two variants and are ready to use:

1. •  Refraction-2D labeling for RGB-fluorescence
   with G-Dye100, G-Dye200 and G-Dye300
2. •  Refraction-2D QPLEX for RGB-IR fluorescence
   with G-Dye100, G-Dye200, G-Dye300 and G-Dye400 .

Refraction-2D™:  100% Made in Germany