Fluorescent Seconadaries

Detection of target proteins

Generally, fluorescent conjugated secondaries are the first choice for target protein detection on Western blots (if the target is not slightly above detection limit, than one must go for ECL).

• • Very easy handling
• • Signal stability of several months
• • Sensitivity in the higher pg range

Immuno Blue HRP-substrate

Detection of target proteins

HRP-conjugated 2^nd antibodies transform Immuno Blue into a fluorescent compound which precipitates and remains stable on the blot (for several months).

• • Short exposure time (10 -100 x shorter than ECL)
• • Long signal stability of several months
• • Sensitivity in the lower pg range

Immuno Pink Detection Kit

Detection of target proteins

Simple and rapid target detection of rapid tests is now also possible in the Western blot. With the Immuno Pink Detection Kit, the effort required for processing a Western blot is significantly reduced, while sensitivity is not reduced.

The Immuno Pink Detection Kit is based on special secondary antibodies coupled with nano gold particles. The kit contains Immuno Pink Block (blocking reagent) and Immuno Pink AK (2nd AK conjugated with nano gold particles).

After 5 min of blocking with Immuno Pink Block, 1st antibody and the Immuno Pink AK are added. After 60 min of incubation, the target can be detected with a simple VIS camera. The sensitivity corresponds to ECL level.

• • Easy handling
• • Sensitivity at ECL level
• • 2^nd antibody already included in the kit
• • Long signal stability of several months
• • No complex washing steps necessary

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