T-Rex Labeling Kit


T-Rex Protein Labeling Kit

Are you searching a more sensitive, better reproducible, and timesaving replacement for post-stains?

The powerful T-Rex protein label combines excellent performance and photostability over a wide pH-range of 2-11. It is therefore ideally suited for high performance visualization of protein samples separated in 2D gels. In contrast to green fluorescence protein stains or labels the red fluorescence of T-Rex causes almost no background.Therefore the signal-to-noise ration of T-Rex is much better compared to most other fluorescence products on the market.

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Western Blotting
T-Rex labeled proteins are detectable after transfer onto Western blot membranes and do not interfere with antibody recognition and mass spectrometry.

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Phosphorylation-/ Glycolysation analysis
The combination of T-Rex label and phospho- or glycostains respectively  allow for powerful identification of differentially regulated phospho/ glycoproteins. The T-Rex labeled sample provides an reliable internal standard (with no specs or gel suffering due to additional staining/ destaining) thereby offering reliable phophorylation/ glycosylation studies.

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Kit content and fluorescence properties
T-Rex kits are ready to use and come as 20 separated vials each for labeling of 50 µg of protein including T-Rex solvent.

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Filter settings: G-Dye300, Cy5, Alexa 647

Exitation max: 650 nm

Emisssion max:  665 nm

 

T-Rex labeling vs. Coomassie® blue staining.

50 μg of protein derived from E. coli were labeled with T-Rex fluorescent label. Another 550 μg of unlabeled protein were added to the sample. The proteins were separated by 2D gel electrophoresis. T-Rex was detected with the Octopus QPLEX imaging device by red epi fluorescence excitation. The gel was stained with Coomassie® blue and then re-imaged by white transmission light.

 

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T-Rex labeling + ProQ® Diamond phosphoprotein staining.

Two samples á 50 μg of protein were labeled with T-Rex fluorescent label. The two samples were separated by 2D gel electrophoresis. The T-Rex labeled sample proteins were detected by the Octopus QPLEX imaging device using epi fluorescence excitation. The two gels were then stained with ProQ® Diamond phosphoprotein gel stain (Thermo Fisher) and then imaged by epi green fluorescence. The two phosphoprotein images were warped (Delta2D, Decodon) using the T-Rex as internal standard. The arrows indicate differentially expressed phosphoproteins of the two samples.

T-Rex Protein Labeling Kit

Product No. Description   Price
PR06  

T-Rex™ Labeling Kit

20 separate vials for 50 µg each

   440,00 EUR

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Workflow

Support

We are happy to provide further information.

Please get in touch with us by phone +49 - 345 -2799 6413 (Mo - Fri 9am - 5pm)

or by email infodyeagnostics.com.

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