EPO Analytics

EPO Protein Analytics

large sample screening, reliable and error-free analysis

The detection of the different genetically or chemically modified EPOs as well as the elevation of sensitivity to detect micro-dosing EPO applications require sophisticated analysis methods. In addition, for laboratories analyzing larger numbers of samples, the tests should be easy to handle and include a higher grade in automation.

We offer a line products along the EPO analysis workflow to meet the requirements of high sensitivity detection, reliability and easy handling.

For standardized rountine EPO diagnostics the ORCA EPO Analyzer provides system integration of powerful electrophoresis and highly sensitive fluorescence and chemiluminescence protein detection.

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Products along the EPO analysis workflow

1. Prior electrophoresis: Fluorescent SEPO standards, added to each sample prior gel electrophoresis, allow for the precise monitoring of the complex workflow as well as the precise RP value determination of every ESA in every lane.

2. Electrophoresis: VELUM Precast Gels for SAR-PAGE, SDS-PAGE and IEF separate up to 40 samples per gel and allow for high sensitivity ESA detection.

3. Blotting: The special design of VELUM Precast Gels, the corresponding VELUM Dry Blotter and VELUM Blotting Kit provide high transfer rates for ESAs to the Western Blot membrane.

4. ESA detection: The ORCA Gel Imager offers both: High sensitivity chemiluminescence and fluorescence detection of large sample gels.

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SEPO fluorescent standards

Sample monitoring and precise RP determination

Prior to electrophoresis, fluorescent SEPO sample standards are added to each sample. The fluorescent detection of the SEPOs allow to carefully monitor the complex workflow (figure 1), as well as to precisely determine the relative position (RP value) of the detected ESAs in every lane. The SEPO-based RP values are independent on electrophoretic differences in sample migration like the “smiley effect” (figure 2).

 

SEPO patent pending technology for monitoring:

  • •  sample load
  • •  separation efficiency of every lane
  • •  protein transfer efficiency of every sample
  • •  relative position (RP value) determination of every sample

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In addition, the protein separation and transfer efficiency (Western blotting) can be easily monitored providing a much higher grade of reliability of the analysis.

SEPOs are in use for daily routine diagnostics by the German WADA laboratory IDAS (Dresden).

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VELUM SAR Precast Gels

VELUM SAR Precast Gels for Sarkosyl-PAGE offer careful separation of up to 40 samples per gel and allow for high sensitivity detection of all ESAs in one analysis.
The combination of VELUM SAR Precast Gels and immunoblotting allows to sensitively discriminate endogenous EPO from exogenous ESAs differing in either the electrical charge (EPO glycoforms) or the molecular weight (EPO analogs/ biosimilars).

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Product No. Description Price
PR247 VELUM SAR Precast Gel 25

Kit of 4 Gels à 25 samples for Sarkosyl-PAGE including buffers and wicks

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PR249 VELUM SAR Precast Gel 40

Kit of 4 Gels à 25 samples for Sarkosyl-PAGE including buffers and wicks

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VELUM SAR Gels are in use for daily EPO routine diagnostics by the German WADA laboratory IDAS (Dresden).

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VELUM SDS-PAGE Precast Gels

VELUM SDS-PAGE Precast Gels offer careful separation of 25 or 52 per gel and allow for high sensitivity detection of all ESAs differing in their molecular weight (EPO analogs/ biosimilars).

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Product No. Description Price
PR227 VELUM Precast SDS Gel 25

Kit of 4 Gels à 25 samples for SDS-PAGE including buffers and wicks

190,00 EUR
PR223 VELUM Precast SDS Gel 40

Kit of 4 Gels à 25 samples for SDS-PAGE including buffers and wicks

215,00 EUR

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VELUM IEF Precast Gels

VELUM IEF Precast Gels offer careful separation of up to 40 samples per gel and allow for high sensitivity detection of all ESAs differing in either the electrical charge (EPO glycoforms).

 

Product No. Description Price
PR VELUM IEF Precast Gel 25

Kit of 4 Gels à 25 samples for IEF including buffers and wicks

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PR VELUM IEF Precast Gel 40

Kit of 4 Gels à 25 samples for IEF including buffers and wicks

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Blotting

The VELUM Dry Blotter is designed for the high quality protein transfer from VELUM Precast Gels. The Blotter is easy to handle, does not require current of additional buffers.

The VELUM Blotting Kits come with precut low fluorescent PVDF membranes and precut filter papers.

Product No. Description Price
PR88 VELUM Dry Blotter 895,00 EUR
PR247 VELUM SAR Blotting Kit 98,00 EUR
PR812B Blot Development Folders

for VELUM Blots

39,00 EUR

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Blot Processing

The BlotCycler from Precision Biosystems allows for automated processing of the Western Blots. We offer washing trays for the blot cycler fitting large size Western Blots (265 x 90 mm) as derived from VELUM Precast Gels.

Product No. Description Price
PR90 Washing Tray for BlotCycler, black

fit blots size 265 x 90mm

890,00 EUR

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ORCA EPO ANALYZER

Integrated workflow - sensitive detection 

The electrophoresis unit of the ORCA EPO Analyzer separates as many as 40 samples per run. For more samples up to three additional electrophoresis units can be added to the system. Fluorescent SEPOs allow to  monitor the protein separation and transfer efficiency in the above imaging unit within seconds.

After finishing the Western Blot procedure, the careful detection of the ESAs is performed again by the highly sensitive imaging unit using chemiluminescence.

ORCA EPO ANALYZER comes with an optional sophisticated 1D gel and blot analysis software (21 CFR Part 11 compliant) but is also compatible for the standard “GASepo” software.

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Support

We are happy to provide further information.

Please get in touch with us by phone +49 - 345 -2799 6413 (Mo - Fri 9am - 5pm)

or by email infodyeagnostics.com.

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