Octoplus QPLEX Imager

Octoplus QPLEX

Octoplus QPLEX acquires large size images of multifluorescent objects such as 2D gels and 2D blots within 20-30 seconds. Sensitivity of fluorescence detection of the Octoplus QPLEX is almost similar to fluorescence scanners. In addition, sensitive chemiluminescence detection enables combined analysis of fluorescence and ECL. Recommended for applications such as Refraction-2D, Saturn-2D, Saturn-2D REDOX, other 2D-DIGE applications as well as HCP analysis.

Product features

  • •  high power 4-color (RGB + NIR) multiplex fluorescence detection system. Almost the same sensitivity as laser scanners but 30x faster.
  • •  homogenous fluorescence and chemiluminescence detection of gels and blots size up to 250 mm (width) and 200 mm (depth).
  • •  robust system with easy and intuitive handling, requires minimal training
  • •  Made in Germany

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Refraction-2D/2D-DIGE/HCP

Image of Refraction-2D/ 2D-DIGE Gel acquired by Octoplus QPLEX

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RGB+NIR Fluorescence/Chemiluminescence Western Blot

Products

Product No. Description Price
 

PR435

 

Octoplus QPLEX

High Power - Large Area

Quadruplex  Fluorescence (RGB+IR) and

High Sensitivity Chemiluminescence Imager

 

 

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PR463

 

UV light transmission module

insertable module

device controlled by Octoplus software

imaging area: 26 x 21 cm or 20 x 20 cm (PR463-S)

 

 

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PR464

 

White light transmission module

insertable module

imaging area: 32 x 23 cm  or 22 x 16 cm (PR464-S)

 

 

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Photo der Innenansicht des Octoplus QPLEX Imagers für RGB + NIR Fluoreszenz, Chemilumineszenz, UV und Weisslichtaufnahmen von großen Flächen

Specifications

homogenous multiplex fluorescence detection area

("publication level)" for 25 x 20 cm

rapid image acquisition

e.g. SPL Western Blots: 0.2 - 1.0 sec.,

VELUM Gold 1D gel: 1-2 sec.

Refraction-2D gel: 10-30 sec.

high power RGB fluorescence detection for e.g.

G-Dye100, G-Dye200, G-Dye300,

Cy2, Cy3, Cy5, ...

high power NIR fluorescence detection for e.g.

G-Dye400, LiCOR CW800, ...

high power ECL detection
sensitive detection of Coomassie stainings (by red power fluorescence)
remote- and hands-on support
low maintenance
Made in Germany

 

Overview of Applications (examples)

Multiplex Fluorescence
quantitative/qualitative

1D gels

size up to 250 x 120 mm

quantitative/qualitative

Western Blots

size up to 250 x 120 mm

quantitative/qualitative

Refraction-2D/2D-DIGE

gels and  Western Blots

e.g. for 24 cm IEF strips

Chemiluminescence
ECL detection

(e.g. for the detection

of low abundant

targets)

 
Vis stains
Coomassie-

staining

(detection by red

power fluorescence)

 

Publications

 

Mengoni, M, Braun, AD, Gaffal, E, Tüting, T. The aryl hydrocarbon receptor promotes inflammation‐induced dedifferentiation and systemic metastatic spread of melanoma cells. Int. J. Cancer. 2020; 112. https://doi.org/10.1002/ijc.33252

 

Paarmann K, Prakash SR, Krohn M, et al. French maritime pine bark treatment decelerates plaque development and improves spatial memory in Alzheimer's disease mice. Phytomedicine. 2019;57:39-48. doi:10.1016/j.phymed.2018.11.033

 

Krohn M, Zoufal V, Mairinger S, Wanek T, Paarmann K, Brüning T, Eiriz I, Brackhan M, Langer O, Pahnke J. Generation and Characterization of an Abcc1 Humanized Mouse Model (hABCC1flx/flx) with Knockout Capability. Molecular Pharmacology. 2019; 96 (2): 138-147. https://doi.org/10.1124/mol.119.115824

 

Kliewe, F., Kaling, S., Lötzsch, H., Artelt, N., Schindler, M., Rogge, H., Schröder, S., Scharf, C., Amann, K., Daniel, C., Lindenmeyer, M.T., Cohen, C.D., Endlich, K. and Endlich, N. (2019), Fibronectin is up‐regulated in podocytes by mechanical stress. The FASEB Journal, 33: 14450-14460. doi:10.1096/fj.201900978RR

 

Frede K, Schreiner M, Baldermann S. Light quality-induced changes of carotenoid composition in pak choi Brassica rapa ssp. Chinensis. Journal of Photochemistry and Photobiology B: Biology 2019; 193: 18-30. https://doi.org/10.1016/j.jphotobiol.2019.02.001

 

Chilcutt NL. Emblica officinalis as a therapeutic supplement in Alzheimer’s disease. Thesis for the Master’s degree in Molecular Biosciences. University of Oslo, June 2019.

 

Shridhar N (2019). Novel vaccination strategies forCD4+ T cell immunotherapy of melanoma. Dissertation.

 

Witzel K, Abu Risha M, Albers P, Börnke F, Hanschen FS. Identification and Characterization of Three Epithiospecifier Protein Isoforms in Brassica oleracea. Front Plant Sci. 2019;10:1552. Published 2019 Dec 19. doi:10.3389/fpls.2019.01552

 

Krohn M, Wanek T, Menet MC, et al. Humanization of the Blood-Brain Barrier Transporter ABCB1 in Mice Disrupts Genomic Locus - Lessons from Three Unsuccessful Approaches. Eur J Microbiol Immunol (Bp). 2018;8(3):78-86. Published 2018 Jul 6. doi:10.1556/1886.2018.00008

 

Genzel, F., Franken, P., Witzel, K. and Grosch, R. (2018), Systemic induction of salicylic acid‐related plant defences in potato in response to Rhizoctonia solani AG 3PT . Plant Pathol, 67: 337-348. doi:10.1111/ppa.12746

 

Witzel K, Üstün S, Schreiner M, Grosch R, Börnke F, Ruppel S. A Proteomic Approach Suggests Unbalanced Proteasome Functioning Induced by the Growth-Promoting Bacterium Kosakonia radicincitans in Arabidopsis. Front Plant Sci. 2017;8:661. doi:10.3389/fpls.2017.00661

 

Witzel K, Buhtz A, Grosch R. Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. Journal of Proteomics. 2017; 169: 215-224. https://doi.org/10.1016/j.jprot.2017.04.008.

 

Genzel F (2017). The molecular basis of the plant-pathogen interaction of potato and Rhizoctonia solani. Dissertation.

 

Faden F, Eschen-Lippold L, Dissmeyer N. Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling. Methods Mol Biol. 2016;1450:247-258. doi:10.1007/978-1-4939-3759-2_20

 

More publications and selected customers you will find here.

Support

We are happy to provide further information.

Please get in touch with us by phone +49 - 345 -2799 6413 (Mo - Fri 9am - 5pm)

or by email infodyeagnostics.com.

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