Refraction-2D™

Refraction-2D™ Labeling Kits

Refraction-2D™ is the leading technology for multiplex-fluorescence 2D gel electrophoresis (also know as differential gel electrophoresis; DIGE). It is based on irreversible binding of new high performing fluorescent G-Dyes (blue G-Dye100, green G-Dye200, red G-Dye300 and infrared G-Dye400) to lysine in proteins.

The G-Dyes are outstandingly photostable and can be re-imaged over periods of months. Due to a spezial low molecular weight G-Dye minimal-labeld proteins can be picked directly from the 2D gel – no futher staining required.

Refraction-2D allows you to...

  • •  run up  to 4 different samples within one 2D gel 
  • •  detect proteins as low abundant as 0.03 ng
  • •  discover even minor but important protein regulation
  • •  identify post translational protein modifications
  • •  pick spots directly from the gel- no post staining required
  • •  Expert support by our 2D coaches

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For Refraction-2D analysis we offer the state-of-the art 2D software Delta2D. Other 2D software, however not as sophisticated, work as well e.g SameSpots (Nonlinear Dynamics), DeCyder (GE Healthcare), PD Quest (Biorad). 

For rapid and sensitive G-Dye image acquisition we recommend the ORCA Fluorescence Imager (RGB) and the Octoplus QPLEX (RGB-IR, ECL). 

Our labeling kits come in two variants and are ready to use:

  1. •  Refraction-2D labeling for RGB-fluorescence
    with G-Dye100, G-Dye200 and G-Dye300
  2. •  Refraction-2D QPLEX for RGB-IR fluorescence
    with G-Dye100, G-Dye200, G-Dye300 and G-Dye400 .

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Refraction-2D™:  100% Made in Germany

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Refraction-2D™ Labeling Kits

 

Prod. No. Description Kit size Price
PR08 Refraction-2D

Labeling Kit

4G

(1x 1.8 nmol)

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PR08G Refraction-2D

Labeling Kit

2x 4G

(2x 1.8 nmol)

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PR09 Refraction-2D

Labeling Kit

12 G

(1 x 5 nmol)

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PR60 Refraction-2D

QPLEX 

Labeling Kit

4G

(1x 1.8 nmol)

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PR61 Refraction-2D

QPLEX 

Labeling Kit

2x 4G

(2x 1.8 nmol)

 

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 PR62 Refraction-2D

QPLEX 

Labeling Kit

12G

(1x 5 nmol)

 

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Image Refraction-2D Labeling Kit for 2D-DIGE analysis of up to 4 protein samples within one analysis.

 

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Refraction-2D™ Kit Content

  • • G-Dye100 - high performance fluorescent dye
  • • G-Dye200 - high performance fluorescent dye
  • • G-Dye300 - high performance fluorescent dye
  • • G-Dye400 - high performance fluorescent dye (for QPLEX kits only)
  • • G-Dye solvent
  • • G-Dye labeling stop solution
  • • G-Dye low retention tips
  • • G-Dye low retention micro centrifuge tubes
  • • G-Dye100 spot picking kit (free for PR09, PR10, PR11, PR12,PR13)
  • • Expert support by our 2D Coaches

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Image Refraction-2D Labeling Kit for 2D-DIGE analysis of up to 4 protein samples within one analysis.

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Refraction-2D™ Kit Quality Assurance

To ensure you a constant quality for your assays all G-Dyes and related Refraction-2D™ kits are subject to stringent quality control. Every batch is checked for sensitivity and labeling efficiency and only kits that succeeded our testing will therefore be delivered to you. All batches are checked periodically in a biweekly fashion throughout the shelf live mentioned on the bag label.

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Refraction-2D™ Labeling Kit - Quality Label

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Refraction-2D™ Publications

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Strohkamp S, Gemoll T, Habermann JK (2016). Possibilities and limitations of 2DE-based analyses for identifying low-abundant tumor markers in human serum and plasma. Proteomics 16: 2519–2532.

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Tesei D, Marzban G, Marchetti-Deschmann M, Tafer  H, Arcalis E, Sterflinger K (2015). Proteome of tolerance fine-tuning in the human pathogen black yeast Exophiala dermatitidis. Journal of Proteomics 128: 39–57.

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Zadražnik T, Hollung K, Egge-Jacobsen W, Meglič V, Šuštar-Vozlič J (2013): Differential proteomic analysis of drought stress response in leaves of common bean (Phaseolus vulgaris L.). Journal of Proteomics 78: 254-272.

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Philipp S (2012): Proteomische Analyse der Zellmembran humaner Erythrozyten als Wirtszellen des Malariaerregers Plasmodium falciparum. Dissertation

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Westman JO, Taherzadeh MJ, Franzén CJ (2012): Proteomic Analysis of the Increased Stress Tolerance of Saccharomyces cerevisiae Encapsulated in Liquid Core Alginate-Chitosan Capsules. PLoS ONE 7(11): e49335.

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Fonseca C, Planchon S, Serra T, Chandler S, Saibo N, Renaut J, Oliveira MM, Batista R (2012): Selection of the best comparator for the risk assessment of GM plants - conventional counterparts vs. negative segregant. Poster presentation

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Gemoll T, Laubert T, Grimme C, Roblick UJ, Habermann JK (2012): Hochauflösende 2D- Gelelektrophorese von Knochengewebsproben. Biospektrum 18 (5): 520-521.

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May C, Brosseron F, Chartowski P, Meyer HE, Marcus K (2012): Differential proteome analysis using 2D-DIGE. Quantitative methods in Proteomics.  Methods in Molecular Biology 893 (2): 75-82.

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Bjarnadóttir SG, Hollung K, Høy M, Bendixen E, Codrea MC, Veiseth-Kent E (2012): Changes in protein abundance between tender and tough meat from bovine Longissimus thoracis muscle assessed by iTRAQ and 2-DE analysis. Journal of Animal Science 90(6): 2035-2043.

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Grimme C, Gemoll T, Habermann J, Seide K, Gerlach UJ (2012): Analysis of protein for the diagnosis of osteitis/osteomyelitis: Initial results. Trauma und Berufskrankheit 14(1): 5-7.

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Smalla KH, Wyneken U (2011):Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis of Brain Synapses. Neuroproteomics 57(3): 95-113.

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Barthel A (2010). Protein analysis and tissue culture of the sex pheromone gland of Lepidoptera. Diplomarbeit am Max-Planck-Institut für Chemische Ökologie (Jena).

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More publications and selected customers you will find here.

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Support

We are happy to provide further information.

Please get in touch with us by phone +49 - 345 -2799 6413 (Mo - Fri 9am - 5pm)

or by email infodyeagnostics.com.

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