March 29 – April 4, 2016

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6. Hands On Proteomic Workshop 
2D-gel electrophoresis (Refraction-2D/ DIGE) incl. mass spec protein identification
 at Vienna University of Technology: Practise how to work with the latest developments in multiplex-fluorescence 2D gel electrophoresis.

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In cooperation with TU Wien, AuPA (Austrian Proteomics Association), BRUKER, and DECODON – we invite you to take part at the hands on workshop “Multiplex fluorescence 2D gel electrophoresis (Refraction-2D/ DIGE) including MS identification using state of the art products.

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Learn more about:

• efficient sample preparation,
• innovative protein labeling,
• horizontal gel electrophoresis,
• fast and reliable analysis of gel images,
• protein indentification by high sensitive MS, and
• more solutions for e.g. redox proteomics and 2D western blots (incl. HCP coverage).

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The workshop includes hands on work, seminars and lectures as well as a introduction in the latest product developments for 2D gel analysis plus tips and recommendations from our expert team.

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Find more information here.

Your NHD Team

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Keywords

Differential protein expression, quantitative proteomics, Refraction-2D, 2D gel electrophoresis, 2D DIGE, VELUM precast gels, ORCA  Gel Electrophoresis, BEO Dry Blotter, VELUM IEF precast gels, horizontal gel electrophoresis

EPO analysis is based on a complex workflow of gel-based protein separation and the subsequent immochemical detection of ESAs. Various modifications as well as the usage of micro-dosing of endogenous EPO can now be detected in high precision within one analysis.

The introduction of special sample-specific standards (SEPOs) allow for step-by-step monitoring as well as for the very precise RP (relative position) determination of EPO bands to clearly distinguish natural and recombinant EPO.

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Learn more about the SEPO technology here.

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Keywords

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SEPO, Blood Doping, Erythropoietin, EPO Micro-Dosing Doping, Endurace, Sarcosyl, SAR PAGE, Electrophoresis, WADA, VELUM SAR, OCRA Gel Electrophoresis, BEO Dry Blotter, VELUM IEF precast gels, horizontal gel electrophoresis, NuPAGE

This article is available in German only.

Improved Sarcosyl PAGE for erythropoietin analysis

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The detection of endurance enhancing erythropoietin (EPO) misusage is currently performed by isoelectric focusing (IEF) based on the profile of altered isoforms of EPO.

Novel Velum SAR precast gels separate EPO isoforms by differences in their molecular masses and introduce a number of advantages …. more

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Keywords

Erythropoietin, EPO, Doping, Endurace, Sarcosyl, SAR PAGE, Electrophoresis, WADA, VELUM SAR, OCRA Gel Electrophoresis, BEO Dry Blotter, VELUM IEF precast gels, horizontal gel electrophoresis, NuPAGE

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Related publications

Schwenke et al.  (2015) Application note

Reichel et al. (2009) Drug Test Anal.

April 7 – 10, 2015

5. Hands On Proteomic Workshop 
2D-gel electrophoresis (Refraction-2D/ DIGE) incl. mass spec protein identification
 at Vienna University of Technology: Practise how to work with the latest developments in multiplex-fluorescence 2D gel electrophoresis.

.

.

In cooperation with TU Wien, AuPA (Austrian Proteomics Association), BRUKER, DECODON and SERVA – we, NH DyeAGNOSTICS GmbH, invite you to take part at the hands on workshop “Multiplex fluorescence 2D gel electrophoresis (Refraction-2D/ DIGE) including MS identification using state of the art products.

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The workshop takes place at the Vienna University of Technology from April 7-10, 2015.  .

Learn more about:

• experimental design
• efficient sample preparation
• multiplex-fluorescence protein labeling
• high performing gel electrophoresis
• rapid but sophisticated analysis of 2D gel images
• protein identification by mass spectrometry

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The workshop includes hands on work, seminars and lectures as well as a introduction in the latest product developments for 2D gel analysis plus tips and recommendations from our expert team.

.

Find more information here.

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For further information please send us an Email.

Your NHD Team

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.

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Keywords

Differential protein expression, quantitative proteomics, Refraction-2D, 2D gel electrophoresis, 2D DIGE, VELUM precast gels, ORCA  Gel Electrophoresis, BEO Dry Blotter, VELUM IEF precast gels, horizontal gel electrophoresis