Smart Protein Layers

Are you using total protein as "loading control" for target normalization of Western Blots? Superb!

But when do you detect the total protein?

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Fig. 1. PVDF membrane loaded with 6x 20 µg total protein from E. coli. Total protein was relabeled with Smart Label Red and detected using an Octoplus QPLEX Imager.

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30-60% of the total protein on a Western blotting membrane is washed away during the several antibody-incubation and washing steps.

Reliable quantification of Western blots therefore requires total protein detection (for normalization) at the point of time the target is detected.

Smart Protein Layers offers real-time total protein detection and many other features.

Find out more.