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Refraction-2D
novel technology
Refraction-2D™ is a novel technology for multiplex fluorescence 2D gel analysis based on irreversible binding of a new set of powerful fluorescent G-Dyes to proteins. Refraction-2D™ introduces…
- advanced, patent pending technology
- non-interference of fluorescence light emission
- 100 % spot matching by bioinformatic processing
- detection sensitivity of 0.1ng
- high dynamic range of 4-5 orders of magnitude
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Refraction-2D
advanced technology
To avoid interference of fluorescence light emission of the dyes bound to the proteins, the G-Dyes are not matched with respect to their electrophoretic mobility. Bioinformatic processing of 2D images allows both the qualitative and quantitative detection of differentially expressed proteins and of post-translational modified proteins, respectively. Therefore, for optimal analysis of multiplex-fluorescence 2D gel electrophoresis, we recommend software suitable for horizontal spot registration (warping) like Delta2D (Decodon GmbH). |
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Refraction-2D
protein labeling
- easy, fast covalent protein labeling with 3 different G-Dyes
- non-saturation labeling for protein expression quantification
- run up to 3 protein samples in one 2D gel
- detect each sample by its specific fluorescence label
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Refraction-2D
work flow
- (1) extract proteins
- (2) label sample with Refraction-2D™ Labeling Kit
- (3) combine samples
- (4) separate protein samples by 2D gel electrophoresis
- (5) scan 2D gels with imaging system/ bioinformatic processing
- (6) analyze protein expression
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Refraction-2D
quantification of protein expression
- 2 samples labeled with fluorescent dyes (e.g. G-Dye 200, G-Dye 300)
- both samples labeled with internal standard (e.g. G-Dye 100)
- analyze protein expression by using appropriate 2D software (e.g. Delta2D/ Decodon, SameSpots/ Nonlinear Dynamics, Proteomweaver/ Bio-Rad).
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